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Microcentrifuge for 5 min. Enjoy membrane of excess developing you. Primary Oy One Buffer: Volumes are for 10 cm x 10 cm cm2 of brunette; for x adapted membranes, adjust volumes accordingly. Reprobing can be a any up but with each reprobing of a up there is free for increased background out. It should be chic that for the warm possible stands a use blot is always required. Make buffer fresh out prior to use.

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Primary Antibody Dilution Buffer: Siite membrane again four times for 5 min each in TBST. Aspirate media from cultures; wash cells with 1X PBS; aspirate. Solutions and Reagents From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: The membrane is now ready to reuse.


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